SERUM LEVEL DETERMINATION OF FLUCONAZOLE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND BIOASSAY

An HPLC method using a reverse phase system, an isocratic mobile phase and 1-phenyl-1,2-ethanediol as internal standard and a weil diffusion bioassay using a strain of Candida pseudotropicalis were compared for the measurement of fluconazole in serum. Both methods permit determin ation of fluconazole in the range from 1 mg/L to 30 mg/L. The correlat ion between both methods was found to be r = 0.89. Both methods are us eful for monitoring the serum level of fluconazole in clinical routine work.