Kc. New et Sd. Rabkin, COEXPRESSION OF 2 GENE-PRODUCTS IN THE CNS USING DOUBLE-CASSETTE DEFECTIVE HERPES-SIMPLEX VIRUS VECTORS, Molecular brain research, 37(1-2), 1996, pp. 317-323
Defective herpes simplex virus (HSV) vectors are an efficient means to
deliver genes to cells of the central nervous system (CNS). Such vect
ors containing two independent transcription units would be extremely
valuable for many studies, including the comparative analysis of promo
ter function and expression of multiple gene products in the CNS. We h
ave constructed a 'double-cassette' vector expressing two easily detec
table marker enzymes, beta-galactosidase (beta-gal) and human placenta
l alkaline phosphatase (AP). Cells infected in vitro, including neuron
s and glia, and in vivo expressed both gene products.