NOVEL HPV TYPES PRESENT IN ORAL PAPILLOMATOUS LESIONS FROM PATIENTS WITH HIV-INFECTION

Patients infected with the human immunodeficiency virus (HIV) often de velop multiple papillomatous lesions of the oral cavity. In the presen t study, a total of 67 biopsies from benign oral lesions were analyzed for the presence of human papillomavirus (HPV) DNA using Southern-blo t hybridization in combination with a polymerase chain reaction design ed to detect all known HPV types, as well as unidentified types. These samples, collected at random from a high-risk population, were subseq uently divided into 57 biopsies originating from patients with confirm ed HIV infection and 10 biopsies from patients with unknown HIV status . Each sample was amplified with 7 different combinations of degenerat e primers. All amplified products were sequenced. HPV DNA sequences we re detected in 67% (45/67) of the samples. HPV 7 (19%) and HPV 32 (28% ) were the predominant HPV types. HPV 32 was present in 2/4 fibromas t ested. Two new HPV types, HPV 72 and HPV 73, were identified in oral w arts with atypia. The complete genomes of these viruses were cloned an d sequenced. Other HPV types detected were HPV 2a, HPV 6b, HPV 13, HPV 16, HPV 18, HPV 55, HPV 59 and HPV 69. (C) 1996 Wiley-Liss, Inc.