SEQUENCE-ANALYSIS OF THE 5'-FLANKING REGION OF THE GENE ENCODING HUMAN N-ACETYLGLUCOSAMINYLTRANSFERASE-III

We have isolated and characterized the immediate (1651 bp) 5'-flanking region of the gene (GnT-III) encoding N-acetylglucosaminyltransferase III (GnT-III) from a human placental genomic library, Analysis of pro moter elements shows a similarity to the 5'-flanking region of murine 1,4-galactosyltransferase. The sequence lacks obvious TATA elements an d CCAAT boxes; however, putative regulatory sites, including 2 potenti al cAMP-response regulatory elements (CRE), 11 insulin-response elemen t consensus sequences (IRE), 7 potential AP-2-binding sites, 2 SP1 con sensus sequences (GC boxes) and 2 sequences similar to the half-palind romic glucocorticoid-responsive element (GRE), are present.