Mb. Viana et al., D-FENFLURAMINE SELECTIVELY RELEASES 5-HT FROM DORSAL RAPHE TERMINALS, Brazilian journal of medical and biological research, 29(5), 1996, pp. 639-642
The aim of this study was to investigate whether D-fenfluramine (FEN)
releases 5-hydroxytryptamine (5-HT) selectively from dorsal raphe (DR)
terminals. Male Wistar rats, 180-200 g, were implanted with microdial
ysis probes in the amygdala (Am; N = 5) and dorsal hippocampus (DH; N
= 6) and 5-HT levels were measured by electrochemical detection. Under
basal conditions, 5-HT levels were approximately 50 and 230 fmol per
30 min sample, in the Am and DH, respectively. FEN (10 mg/kg, ip) prod
uced a 3-4-fold increase in 5-HT release in the Am, but not in the DH.
Since the Am is mainly innervated by DR fibers while the DH receives
5-HT input chiefly from the median raphe (MR), the present results sup
port the view that FEN selectively releases 5-HT from DR terminals.