A. Daugschies, INVESTIGATIONS INTO THE PRODUCTION AND FUNCTION OF LEUKOTRIENES DURING HISTOTROPIC DEVELOPMENT OF OESOPHAGOSTOMUM-DENTATUM, Parasitology research, 82(5), 1996, pp. 416-422
The dynamics of production and excretion of leukotrienes by parasitic
larvae of Oesophagostomum dentatum and their role for the development
of the larvae were studied. Larvae were cultured in vitro to the fourt
h stage (L4). Leukotriene B4 (LTB4) was detected in homogenates and in
supernatant of larvae, with the homogenate-protein-based values stead
ily decreasing during development. The homogenate-protein-based values
of peptidyl leukotrienes (pepLT) remained fairly stable in both homog
enates and supernatants, whereas the worm-count-based pepLT values inc
reased significantly. The addition of diethylcarbamazine (DEC) to the
culture medium straight from the beginning of culturing (12.8 or 25.5
mmol/l) reversibly hampered growth and development to L4. Application
of DEC at 12.8 mmol/l beginning on day 13 of in vitro cultivation exer
ted no significant effect on further development to L4. LTB4 appeared
to counteract the inhibition of development by DEC. The results of thi
s study indicate that endogenous LTs participate in regulation of the
growth and development of O. dentantum.