CRYPTOSPIROLEPINE, A UNIQUE SPIRO-NONACYCLIC ALKALOID ISOLATED FROM CRYPTOLEPIS-SANGUINOLENTA

From the alkaloidal fractions of the West African plant Cryptolepis sa nguinolenta (Asclepiadaceae), two alkaloids were purified: one was ide ntified as the known indoloquinoline alkaloid cryptolepine [1]. A seco nd, novel alkaloid was shown to have an empirical formula of C34H24N4O based on exact mass measurement. Through the concerted application of a series of homonuclear and inverse-detected 2D nmr experiments, the structure of the second alkaloid was established as a spiro-nonacyclic alkaloid, cryptospirolepine. One portion of the structure of cryptosp irolepine [2] may be biogenetically derived from cryptolepine [1].