Jm. Dominguez et al., PRETREATMENT OF SUGAR-CANE BAGASSE HEMICELLULOSE HYDROLYSATE FOR XYLITOL PRODUCTION BY YEAST, Applied biochemistry and biotechnology, 57-8, 1996, pp. 49-56
A total of six known xylitol-producing yeast strains were screened for
production of xylitol from xylose. Candida sp. 11-2 proved to be the
best producer. It was chosen to study its ability to produce xylitol f
rom hemicellulose hydrolysate derived from sugar cane bagasse. The hyd
rolysate was prepared by dilute sulfuric acid (2-3% [w/v]) hydrolysis,
with a high-solid, low-liquid ratio followed by leaching. Owing to th
e inhibitors present in the hydrolysate, different treatments were stu
died to overcome its effect. In order to reach higher xylitol producti
vity, treated hydrolysates were concentrated by vacuum evaporation in
rotavapor to provide a higher initial xylose concentration. After trea
tment, Candida sp. 11-2 was able to ferment xylose in hemicellulose hy
drolysate to produce xylitol.