Hydrogen is a clean energy alternative to the fossil fuels, the main s
ource of greenhouse gas emissions. We developed a stable system for th
e conversion of solar energy into hydrogen using photosynthetic microo
rganisms. Our system consists of the following three stages: 1. Photos
ynthetic starch accumulation in green microalgae (400 L x2); 2. Dark a
naerobic fermentation of the algal starch biomass to produce hydrogen
and organic compounds (155 L x2); and 3. Further conversion of the org
anic compounds to produce hydrogen using photosynthetic bacteria (thre
e types of reactors, parallel plate, raceway, and tubular). We constru
cted a test plant of this process at Nankoh power plant of Kansai Elec
tric Power Company in Osaka, Japan, and carried out a series of tests
using CO2 obtained from a chemical. absorption pilot-plant. The photob
iological hydrogen production process used a combination of a marine a
lga, Chlamydomonas sp. MGA 161 and marine photosynthetic bacterium, Rh
odopseudomonas sp. W-1S. The dark anaerobic fermentation of algal star
ch biomass was also investigated. Sustained and stable starch accumula
tion, starch degradation in the algal cell, and hydrogen production fr
om algal fermentation and photosynthetic bacteria in the light were de
monstrated during several experiments.