Bulking and foaming in activated sludge have been associated with fila
mentous overgrowth. Filamentous Nocardia amarae and nonfilamentous Pse
udomonas auruginosa were cultured using fatty acids (C2-C24) as the so
le carbon. N. amarae could utilize all acids tested for growth, wherea
s P. auruginosa hardly grew on acids with 12 or more carbons. Maximum
specific growth rate and saturation constant of N. amarae on C24, at 0
.048 h(-1) and 1.520 g COD/L, respectively,were much lower than that o
f P. auruginosa, showing that N. amarae had a relatively stronger affi
nity for long-chain fatty acids. N. amarae was competitive in activate
d sludge processes that receive sewage containing a high proportion of
long-chain fatty acids, oils, and fats.