COMPUTERIZED AMPEROMETRIC DETECTION FOR C APILLARY ELECTROPHORESIS

A computerized end-column amperometric detector for capillary zone ele ctrophoresis is constructed. The microelectrode is positioned at the e nd of 25 mu m i.d. capillary by micromanipulator directly. The electro de potential is controlled and the signal is sampled by the microcompu ter. Application of the system to the separation and detection of NE, IP and CAT is demonstrated. This system is also applied to investigate the field-amplified injection in capillary electrophoresis with amper ometric detection and the concentration limits of 0.45 similar to 0.65 nmol/L for DA, NE, E and IP are achieved.