We report the purification of a Ras-GTPase-activating protein (GAP)-bi
nding protein, G3BP, a ubiquitously expressed cytosolic 68-kDa protein
that coimmunoprecipitates with GAP. G3BP physically associates with t
he SH3 domain of GAP, which previously had been shown to be essential
for Ras signaling. The G3BP cDNA revealed that G3BP is a novel 466-ami
no-acid protein that shares several features with heterogeneous nuclea
r RNA-binding proteins, including ribonucleoprotein (RNP) motifs RNP1
and RNP2, an RG-rich domain, and acidic sequences. Recombinant G3BP bi
nds effectively to the GAP SH3 domain G3BP coimmunoprecipitates with G
AP only when cells are in a proliferating state, suggesting a recruitm
ent of a GAP-G3BP complex when Ras is in its activated conformation.