Ks. Lin et Nn. Chuang, ANIONIC GLUTATHIONE S-TRANSFERASES IN SHRIMP EYES, Comparative biochemistry and physiology. B. Comparative biochemistry, 105(1), 1993, pp. 151-156
1. Two anionic isoenzymes of glutathione transferases (EC 2.5.1.18), Q
I and QII, have been purified from the eyes of the shrimp Penaeus japo
nicus by using a combination of S-hexylglutathione affinity column chr
omatography and Mono-Q fast protein liquid chromatography (f.p.l.c.).
2. Both QI and II glutathione S-transferases are homodimers. They show
similarity in substrate specificities and pH optima, but not in isoel
ectric points. 3. QI is distinct from QII by anion-exchange f.p.l.c.,
reverse-phase h.p.l.c. chromatography and amino acid sequencing analys
is. QI has N-terminal amino acid sequences homologous to mu glutathion
e S-transferase, whereas QII is homologous to theta glutathione S-tran
sferases.