MODULATION OF MICROSOMAL CYTOCHROME-P450 BY SCUTELLARIAE RADIX AND GENTIANAE-SCABRAE RADIX IN RAT-LIVER

Authors
KANG JJ CHEN YC KUO WC CHEN T CHENG YW KUO ML UENG TH
Citation
Jj. Kang et al., MODULATION OF MICROSOMAL CYTOCHROME-P450 BY SCUTELLARIAE RADIX AND GENTIANAE-SCABRAE RADIX IN RAT-LIVER, The American journal of Chinese medicine, 24(1), 1996, pp. 19-29
Citations number
27
Categorie Soggetti
Medicine, General & Internal
Journal title
The American journal of Chinese medicine → ACNP
ISSN journal
0192415X
Volume
24
Issue
1
Year of publication
1996
Pages
19 - 29
Database
ISI
SICI code
0192-415X(1996)24:1<19:MOMCBS>2.0.ZU;2-8
Abstract
The present study has determined the effects of Scutellariae Radix (Hu angqin) and Gentianae scabrae Radix (Longdan) on liver microsomal cyto chrome P450 (P450)-dependent mono-oxygenases using rats pretreated wit h crude extracts of medicinal herbs. Scutellariae Radix resulted in a 53% decrease of pentoxyresorufin O-dealkylase activity in liver micros omes. In contrast, Gentianae scabrae Radix caused a 50% increase of be nzo(a)pyrene hydroxylase activity. Immunoblotting analysis of liver mi crosomes revealed that Scutellariae Radix induced and suppressed the l evels of P450 1A and 2B proteins, respectively. Scutellariae and Genti anae scabrae Radixes had no effects on microsomal aniline hydroxylase activity and P450 2E1 protein.