PREPARATION AND CHARACTERIZATION OF A MONOCLONAL-ANTIBODY SPECIFIC FOR THE BETA-AGONIST CLENBUTEROL

Authors
PETRUZZELLI E IUS A BERTA S DOVIS M ALBERTINI A
Citation
E. Petruzzelli et al., PREPARATION AND CHARACTERIZATION OF A MONOCLONAL-ANTIBODY SPECIFIC FOR THE BETA-AGONIST CLENBUTEROL, FOOD AND AGRICULTURAL IMMUNOLOGY, 8(1), 1996, pp. 3-10
Citations number
15
Categorie Soggetti
Immunology,"Food Science & Tenology","Agriculture Dairy & AnumalScience
Journal title
FOOD AND AGRICULTURAL IMMUNOLOGY → ACNP
ISSN journal
09540105
Volume
8
Issue
1
Year of publication
1996
Pages
3 - 10
Database
ISI
SICI code
0954-0105(1996)8:1<3:PACOAM>2.0.ZU;2-8
Abstract
A monoclonal antibody was obtained from a Balb/c mouse immunized with a conjugate prepared by the direct coupling of diazotized clenbuterol to bovine serum albumin. The antibody, characterized by gamma(1), kapp a- isotype and an affinity constant of 3.5 x 10(8) l mol(-1), is highl y specific as its cross-reactivity to structurally related molecules i s less than 1%. A competitive ELISA for the detection of clenbuterol i n horse and human urine was developed using this antibody.