Dt. Lowery et al., EFFECT OF NEEM ON THE GROWTH AND DEVELOPMENT OF THE OBLIQUEBANDED LEAFROLLER, CHORISTONEURA-ROSACEANA, Entomologia experimentalis et applicata, 79(2), 1996, pp. 203-209
Neem, Azadirachta indica A. Juss. (Meliaceae), seed oil (NSO) added to
meridic diet at concentrations as low as 0.016% reduced pupation and
prevented adult eclosion of obliquebanded leafroller, Choristoneura ro
saceana (Harris) (Lepidoptera: Tortricidae). At a rate of 0.0016%, NSO
reduced the fitness of C, rosaceana, resulting in longer developmenta
l times, lower adult eclosion rates, and reduced egg production compar
ed with controls. The neem insecticide Margosan-O (TM) produced compar
able results based on concentrations of the most biologically active c
onstituent, azadirachtin. Pupation was completely inhibited at concent
rations of 0.25% and 1.0% for larvae exposed at 5th or 6th instar, res
pectively; rates as low as 0.016% reduced pupal weights and adult eclo
sion rates. For larvae transferred to treated diet at 5th instar, phys
ical abnormalities in the wings of adults occurred at a rate of 0.004%
NSO and increased with increasing treatment rates. NSO at concentrati
ons as high as 2.0% was not antifeedant to neonate larvae, based on 24
and 48 h choice test bioassays, when incorporated into a meridic diet
.