GS28, A 28-KILODALTON GOLGI SNARE THAT PARTICIPATES IN ER-GOLGI TRANSPORT

Little is known about the integral membrane proteins that participate in the early secretory pathway of mammalian cells. The complementary D NA encoding a 28-kilodalton protein (p28) of the cis-Golgi was cloned and sequenced. The protein was predicted to contain a central coiled-c oil domain with a carboxyl-terminal membrane anchor. An in vitro assay for endoplasmic reticulum-Golgi transport was used to show that p28 p articipates in the docking and fusion stage of this transport event. B iochemical studies established that p28 is a core component of the Gol gi SNAP receptor (SNARE) complex.