A LIGHT-SCATTERING INVESTIGATION OF THE SODIUM DODECYL SULFATE-LYSOZYME SYSTEM

Dynamic and static light scattering measurements have been made on lys ozyme solutions at 25 degrees C in 0.1 M phosphate buffer, pH 6.88, in the presence of sodium dodecyl sulfate (SDS). The concentration of SD S has been varied over a wide range (0-0.2 g/ml). It is found that und er these conditions both SDS micelles and lysozyme(Lys)-SDS complexes coexist. Addition of lysozyme at constant SDS concentration leads to f ormation of more lysozyme-SDS complex. The translational diffusion coe fficient of the complex increases with SDS concentration. It is also f ound that two lysozyme molecules are associated in the protein-surfact ant complex. (C) 1996 American Institute of Physics.