N. Kameoka et al., HEPATIC ADENINE-NUCLEOTIDES AND DNA-SYNTHESIS DURING THE REGENERATIVEAND ATROPHIC PROCESS OF THE LIVER LOBES AFTER SELECTIVE PORTAL-VEIN LIGATION, European surgical research, 28(3), 1996, pp. 212-221
Selective portal vein occlusion prior to aggressive hepatic resection
is now an alternative way to decrease postoperative morbidity and mort
ality rates. However, the detailed changes in the hepatic energy statu
s and DNA synthesis rate in both portal vein ligated (PVL) and nonliga
ted (PVNL) lobes of the liver are not dear. In rats, the portal branch
that supplies 70% of the liver volume was ligated, and changes in art
erial ketone body ratio (AKBR), liver weight, histology, DNA synthesis
rate and adenine nucleotides of the PVL and PVNL liver lobes were det
ermined before and 1, 2, 4 and 7 days after portal vein ligation, and
compared with those in sham-operated rats. The weight of the PVL lobes
decreased, while that of the PVNL lobes increased depending on time.
The DNA synthesis rates of the PVNL lobes were significantly higher th
an those in sham-operated control fiver during the first 4 days with t
he maximal value on the 2nd day, while those of PVL lobes were essenti
ally similar to the control values, Energy charge (EC) in both PVL and
PVNL lobes significantly decreased on day I and recovered gradually,
but with less extent in the regenerating PVNL lobes. The concentration
s of total adenine nucleotides (TAN) in both the PVL and PVNL lobes we
re essentially similar during the first 2 days, but became significant
ly lower in PVL lobes after day 4. A decrease in EC preceded an increa
se in DNA synthesis only in the PVNL lobes, in contrast to the PVL lob
es. Mitosis of hepatocytes on day 2 and subsequently enlarged lobules
with an increased number of hepatocytes were histologic features in th
e PVNL liver. The AKBR was not correlated with hepatic energy charge o
f the liver. In conclusion, PVNL liver regenerates preceded by a decre
ase in EC and a subsequent increase in DNA synthesis keeping TAN const
ant, while PVL liver becomes atrophic, with a similar change in EC of
the PVNL liver but ultimately decreased TAN without any change in DNA
synthesis. AKBR is not a parameter reflecting the hepatic EC after por
tal branch ligation.