DETECTION OF IMMUNOGLOBULINS ON BACTERIAL SURFACE BY LASER FLOW-CYTOMETRY - ANALYSIS BETWEEN HAEMOPHILUS-INFLUENZAE TYPE-B AND VIBRIO-CHOLERAE O1 OF HEALTHY MOTHER-FULL TERM NEWBORN SERA

The identification of human IgG immunoglobulins on the surface of Vibr io cholerae O1, and Haemophilus influenzae type b microorganisms was a ssessed via a flow cytometric technique, A group of 31 healthy mother- full term newborn duo sera from a non-endemic cholera area was assayed , The sera of mothers and full-term newborns against both microorganis ms were compared, The mean fluorescent intensity of the samples was no t different at the 0.05 significance level by paired t-test, On the ot her hand, the immunoglobulins of newborn and mothers for V. cholerae O 1 was notably lower when compared with H. influenzae type b microorgan isms (p < 0.05 by paired t-test, t = -5.570 for mothers' sera, and t = -7.496 for the sera of the newborns), These data provide circumstanti al evidence that LFC technique would be useful on bacteria-related ser ology.