A SIMPLE TECHNIQUE FOR DIRECT TRANSFORMATION AND REGENERATION OF THE DIPLOID LEGUME SPECIES LOTUS-JAPONICUS

The diploid legume species Lotus japonicus was transformed using a dis armed Agrobacterium strain. By inoculating the cotyledon attachment si te, we took advantage of the regenerative capability of the plant whic h spontaneously regenerated transformed shoots. Some of these shoots w ere propagated as transgenic plants and later transferred to the glass house to produce seeds. Seeds were germinated and yielded non-chimeric , transgenic stably modified Lotus plants.