HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF CREATININE IN SERUM, AND A CORRELATION OF THE RESULTS WITH THOSE OF THE JAFFE AND ENZYMATIC METHODS

A high-performance liquid chromatographic method for the determination of creatinine in control sera is reported, based on its separation fr om deproteinated serum components on the ion-exchange material HEMA Bi o 1000 SB and ultraviolet detection at 230 nm. Groups of eleven to fou rteen samples of human serum and several control materials were simult aneously analysed by the Jaffe, enzymic ultraviolet and enzymic peroxi dase aminophenazone methods. Another group (52-115 sera) was analysed for correlations with spectrophotometric methods. The precision of the chromatographic method ranges between 2.0 and 1.0% (relative standard deviation) for serum creatinine concentrations of 115.1 to 471 mumol/ l, respectively. A very good accuracy was found in analyses of referen ce materials Kontrollogen-L and -LP. Some results of analyses of the o ther control sera were higher and the other lower than those obtained by the Jaffe and enzymic methods, because both interferences and enzym e inhibitors were encountered. Correlations between the chromatographi c and spectrophotometric methods were good.