THE UTILITY OF FLUORESCENT IN-VIVO REPORTER GENES IN MOLECULAR CARDIOLOGY

Citation
Pa. Doevendans et al., THE UTILITY OF FLUORESCENT IN-VIVO REPORTER GENES IN MOLECULAR CARDIOLOGY, Biochemical and biophysical research communications, 222(2), 1996, pp. 352-358
Citations number
25
Categorie Soggetti
Biology,Biophysics
ISSN journal
0006291X
Volume
222
Issue
2
Year of publication
1996
Pages
352 - 358
Database
ISI
SICI code
0006-291X(1996)222:2<352:TUOFIR>2.0.ZU;2-C
Abstract
In vivo reporter genes can be used in different ways in molecular card iology. In this paper studies are presented using the green fluorescen t protein and one of its mutants, S65T-GFP, as in vivo reporter genes. With this new molecular tool we studied cell type specificity of the murine ventricular myosin light chain 2 promoter, positive cell identi fication prior to parch clamp procedures, and the use of fluorescence activated cell sorting of transiently transfected mammalian cells. (C) 1996 Academic Press, Inc.