The amino acid sequence of the rat 60S ribosomal subunit protein L14 w
as deduced from the sequence of nucleotides in two recombinant cDNAs.
Ribosomal protein L14 has 213 amino acids (the NH2-terminal methionine
is removed after translation of the mRNA); the molecular weight is 23
,193. Hybridization of the cDNA to digests of nuclear DNA suggests tha
t there are 6 to 8 copies of the L14 gene. The mRNA for the protein is
about 800 nucleotides in length. Rat L14 is related to a number of pr
eviously unidentified ribosomal proteins from other eukaryotes but not
to any from archaebacteria or eubacteria. The carboxyl-terminal amino
acid sequences of rat L14 and of chicken histone H1 are related. Muta
tion of the Drosophila melanogaster, homolog of rat L14 causes a sever
e Minute phenotype. (C) 1996 Academic Press, Inc.