ZOO-FISH ANALYSIS IN THE HORSE, EQUUS-CABALLUS, DETECTS REGIONS HOMOLOGOUS TO HUMAN-CHROMOSOME-3 AND HUMAN-CHROMOSOME-14

Chromosomal homologies between human chromosomes 3 and 14 and the hors e karyotype have been established, by using human chromosome-specific DNA libraries in heterologous chromosome painting experiments called Z OO-FISH. Biotin-labelled DNA libraries from flow-sorted human chromoso mes 3 and 14 were used as probes for fluorescence in-situ hybridizatio n (FISH) on horse chromosomes. Human DNA library 3 hybridized to equin e chromosomes 16 and 19, identifying and delineating two segments of h omology. Human DNA library 14 hybridized to equine chromosomes 1 and 2 4 or 25.