PURIFICATION OF PEANUT LECTIN USING GUAR GUM AS AN AFFINITY LIGAND

Affinity precipitation is a convenient purification approach which com bines the advantages of precipitation with selectivity of affinity int eractions. The main limitation of the technique at present is the cost of affinity ligands and matrix. In this work, peanut lectin was isola ted by affinity precipitation using guar gum linked alginate. Both mat rix as well as affinity ligand were inexpensive in nature. The recover ed lectin (46 mg per 100 g seeds) purified by this single-step procedu re appeared as a single band of M(r) 29000 on SDS-polyacrylamide gel e lectrophoresis.