Affinity precipitation is a convenient purification approach which com
bines the advantages of precipitation with selectivity of affinity int
eractions. The main limitation of the technique at present is the cost
of affinity ligands and matrix. In this work, peanut lectin was isola
ted by affinity precipitation using guar gum linked alginate. Both mat
rix as well as affinity ligand were inexpensive in nature. The recover
ed lectin (46 mg per 100 g seeds) purified by this single-step procedu
re appeared as a single band of M(r) 29000 on SDS-polyacrylamide gel e
lectrophoresis.