PRODUCTION OF SANGUINARINE BY ELICITED PLANT-CELL CULTURE .2. FURTHERNUTRITIONAL ASPECTS

Papaver somniferum cell suspension cultures carried out in shake flask s were elicited with chitin under batch and nutrient feeding condition s to increase sanguinarine production. Batch cultures yielded maximum production of 270 mg l(-1) upon elicitation during exponential growth phase at extracellular phosphate depletion. The addition of concentrat ed medium solutions prior to elicitation of stationary phase cultures allowed a modest increase in production to 350 mg l(-1). Further optim ization of the nutrient solution yielded production of 575 mg l(-1). E xamination of the composition of this nutrient solution showed an abso lute requirement of elicited cultures for the presence of carbohydrate s, phosphate and sulfate during fedbatch additions for maximum sanguin arine production.