NICOTINIC RECEPTOR-MEDIATED CA2+ MOBILIZATION AND CATECHOLAMINE SECRETION IN CHICK ADRENAL CHROMAFFIN CELLS

Authors
TERAOKA H MATSUZAWA R MARUYAMA Y HIRAGA T OHGA A
Citation
H. Teraoka et al., NICOTINIC RECEPTOR-MEDIATED CA2+ MOBILIZATION AND CATECHOLAMINE SECRETION IN CHICK ADRENAL CHROMAFFIN CELLS, Proceedings of the Japan Academy. Series B Physical and biological sciences, 72(3), 1996, pp. 52-55
Citations number
19
Categorie Soggetti
Multidisciplinary Sciences",Biology
Journal title
Proceedings of the Japan Academy. Series B Physical and biological sciences → ACNP
ISSN journal
03862208
Volume
72
Issue
3
Year of publication
1996
Pages
52 - 55
Database
ISI
SICI code
0386-2208(1996)72:3<52:NRCMAC>2.0.ZU;2-J
Abstract
We reinvestigated the involvement of the nicotinic-acetylcholine recep tor in catecholamine secretion from chick adrenal chromaffin cells, Ni cotine (10(-8)-10(-4)M), as well as methacholine, caused a dose-depend ent increase in the cytosolic concentration of Ca2+ and catecholamine secretion from freshly isolated adrenal chromaffin cells. The effects of acetylcholine (10(-4)M) were effectively inhibited by both hexameth onium (10(-4)M) and atropine (10(-5)M), and almost completely blocked by both antagonists. The results suggest the existence of functional n icotinic receptors, which mediate Ca2+ mobilization and catecholamine secretion, in chromaffin cells of chick adrenal glands.