Oas. Abdurahman et Am. Saad, CHARACTERIZATION OF CAMEL LEUKOCYTES BY FLOW-CYTOMETRY AND MICROSCOPIC EVALUATION OF GRANULOCYTE PHAGOCYTOSIS OF FLUORESCENT BACTERIA, Journal of veterinary medicine. Series A, 43(2), 1996, pp. 111-117
Peripheral blood was obtained from four lactating camels (Camelus bact
rianus) and flow cytometry was used to characterize cell populations.
The ability of the granulocytes to engulf fluorescent bacteria was stu
died in vitro using fluorescence microscopy. Three clusters of blood c
ells (mononuclear cells, neutrophils and eosinophils) were identified
by flow cytometry and fluorescent microscopy. Milk, on the other hand,
was dominated by cell fragments and no distinct cluster formation was
found. The mean yield of blood granulocytes and monocytes isolated on
Ficoll gradient was 92.2 +/- 5.4 % and 7.9 +/- 5.7 %, respectively. C
ell viability was 95 %. The mean percentage of phagocytic cells was 71
.8 +/- 5.9 % at 10 min and increased to 97.3 +/- 0.5 % at 60 min when
observation was terminated. The average number of bacteria per phagocy
te was 8.7 +/- 2.1 and 13.1 +/- 0.9 at 10 and 60 min incubation time.