DETERMINATION OF CHOLESTEROL IN FAT AND MUSCLE OF PIG BY HPLC AND CAPILLARY GAS-CHROMATOGRAPHY WITH SOLVENT VENTING INJECTION

Two methods for determination of cholesterol in fat and muscle of pig were evaluated: extraction with chloroform:methanol (2:1, nu/nu) follo wed by saponification (method 1) and direct saponification (method 2), HPLC and GC were used to determine cholesterol concentrations, GC ana lysis was performed with a capillary column of 100 mu m using a PTV in jector in the modes of cold split and solvent venting. Cholesterol was analyzed without derivatization. Both methods of extraction did not p resent significant differences (p > 0.01), Sample analysis by GC with solvent venting injection and HPLC showed the lowest % r.s.d, but GC i n the cold split mode allowed to obtain a shorter analysis time, Chole sterol concentrations obtained by HPLC mere not statistically differen t from the results obtained by GC with solvent venting injection and w ere slightly lower than those previously reported. Cholesterol concent rations in fat and muscle tissues respectively ranged from 52 to 77 mg /100 g and from 55 to 65 mg/100 g.