CHANGES IN BETA(1)-ADRENOCEPTOR AND BETA(2)-ADRENOCEPTOR MESSENGER-RNA LEVELS IN ALVEOLAR TYPE-II CELLS DURING CULTIVATION

Authors
ISOHAMA Y MATSUO T KAI H TAKAHAMA K MIYATA T
Citation
Y. Isohama et al., CHANGES IN BETA(1)-ADRENOCEPTOR AND BETA(2)-ADRENOCEPTOR MESSENGER-RNA LEVELS IN ALVEOLAR TYPE-II CELLS DURING CULTIVATION, Biochemistry and molecular biology international, 36(3), 1995, pp. 561-568
Citations number
15
Categorie Soggetti
Biology
Journal title
Biochemistry and molecular biology international → ACNP
ISSN journal
10399712
Volume
36
Issue
3
Year of publication
1995
Pages
561 - 568
Database
ISI
SICI code
1039-9712(1995)36:3<561:CIBABM>2.0.ZU;2-D
Abstract
Alveolar type II cells, the progenitor of type I epithelial cells, syn thesize and secrete pulmonary surfactant. In this study, we determined the levels of mRNA for beta(1)- and beta(2)-adrenoceptor in cultured type II cells using a quantitative RNase protection assay. The mRNA le vels for beta(1)- and beta(2)-adrenoceptors in the freshly isolated ce lls were 0.51 +/- 0.07 and 1.92 +/- 0.19 amol/mu g total cellular RNA, respectively. In the cells cultured for over 2 days, only beta(1)-adr enoceptor mRNA level was remarkably reduced. These results suggest tha t each mRNA expression may be separately regulated in cultured alveola r type II cells.