PHOSPHATIDYLSERINE SYNTHESIS IN PHORBOL ESTER TREATED GLIOMA C6 CELLS

Phosphatidylserine (PS) synthesis was studied in glioma C6 cells with [C-14]serine and in the presence or absence of the phorbol ester, 12-O -tetradecanoylphorbol 13-acetate (TPA). It was found that incubation o f the cells with 10 nM or 100 nM TPA for 1h inhibited PS formation by 30% and 60%, respectively. Long-term (18h) treatment of the cells with 100 nM TPA diminished PS formation and further addition of TPA to dow n-regulated cells did not affect PS synthesis. The data show that the changes in PS synthesis can be associated with alterations in morpholo gy of cell and the actin cytoskeleton organization. The role of protei n kinase C in this process is discussed.