MUSHROOM TYROSINASE HAS AN ASCORBATE OXIDASE ACTIVITY

Authors
ROS JR RODRIGUEZLOPEZ JN VARONCASTELLANOS R GARCIACANOVAS F
Citation
Jr. Ros et al., MUSHROOM TYROSINASE HAS AN ASCORBATE OXIDASE ACTIVITY, Biochemistry and molecular biology international, 36(2), 1995, pp. 301-309
Citations number
35
Categorie Soggetti
Biology
Journal title
Biochemistry and molecular biology international → ACNP
ISSN journal
10399712
Volume
36
Issue
2
Year of publication
1995
Pages
301 - 309
Database
ISI
SICI code
1039-9712(1995)36:2<301:MTHAAO>2.0.ZU;2-H
Abstract
The reaction between mushroom tyrosinase and L-ascorbic acid was studi ed by oxymetric assays and evidence pointing to ascorbate oxidase acti vity of this enzyme has been obtained. The activity is clearly linear to enzyme concentration and the Michaelis constant for L-ascorbic acid has a value of 2.69 +/- 0.11 mM. Maximum activity is obtained at pH 7 .5. A possible reaction mechanism, which is based on the different enz ymatic forms of tyrosinase, is also presented.