EXPRESSION OF THE 2 IDURONATE-2-SULFATASE CDNAS

The iduronate-2-sulfatase (LDS) is a lysosomal enzyme that acts on sul phate groups on C-2 positions of the iduronic acid residues of the muc opolysaccharides heparan sulphate and dermatan sulphate. Recently, we described in mouse two IDS mRNAs: the first or canonic (MTA16), highly homologous to the human counterpart, the second or novel (MTA13), com pletely divergent from the canonic in the 3' region. In this study, by reverse transcriptase polymerase chain reaction (RT-PCR) we analyzed the expression of the two mRNA transcripts for the IDS gene in murine tissues, in various human cell-lines and in cells from some Hunter pat ients.