PARTIAL COLOCALIZATION OF GLUCOCORTICOID AND MINERALOCORTICOID RECEPTORS IN DISCRETE COMPARTMENTS IN NUCLEI OF RAT HIPPOCAMPUS NEURONS

Citation
B. Vansteensel et al., PARTIAL COLOCALIZATION OF GLUCOCORTICOID AND MINERALOCORTICOID RECEPTORS IN DISCRETE COMPARTMENTS IN NUCLEI OF RAT HIPPOCAMPUS NEURONS, Journal of Cell Science, 109, 1996, pp. 787-792
Citations number
23
Categorie Soggetti
Cell Biology
Journal title
ISSN journal
00219533
Volume
109
Year of publication
1996
Part
4
Pages
787 - 792
Database
ISI
SICI code
0021-9533(1996)109:<787:PCOGAM>2.0.ZU;2-F
Abstract
The glucocorticoid receptor and the mineralocorticoid receptor are hor mone-dependent transcription factors, They regulate the excitability o f rat hippocampus CA1 neurons in a coordinated fashion, We studied the spatial distribution of these transcription factors in nuclei of CA1 neurons by dual labeling immunocytochemistry and confocal microscopy, combined with novel image restoration and image analysis techniques, W e found that both receptors are concentrated in about one thousand clu sters within the nucleus, Some clusters contain either mineralocortico id receptors or glucocorticoid receptors, but a significant number of clusters contains both receptors. These results indicate that the two receptor types are targeted to specific compartments in the nucleus, T he coordinated action of the glucocorticoid and mineralocorticoid rece ptor on gene expression may be established in a specific set of nuclea r domains that contain both receptors.