G. Talaska et al., CARCINOGEN-DNA ADDUCTS IN EXFOLIATED UROTHELIAL CELLS - TECHNIQUES FOR NONINVASIVE HUMAN MONITORING, Environmental health perspectives, 99, 1993, pp. 289-291
Detection of carcinogen-DNA adducts m DNA from exfoliated urothelial c
ells from animals and humans exposed to potential environmental carcin
ogens is described. In an animal model, 4-aminobiphenyl-DNA adducts we
re detected, and the shape of the dose-response curve was related to t
he levels of 4-aminobiphenyl-hemoglobin adducts. In a human study, fiv
e distinct adducts were two to nine times higher in smokers than in no
nsmokers. The association of four adduct measures with smoking was cor
roborated by significant correlations with levels of 4-aminobiphenyl-h
emoglobin adducts, type and number of cigarettes smoked, and/or urinar
y mutagenicity. One adduct seemed chromatographically similar to N-(de
oxyguanosin-8-yl)-4-aminobiphenyl. This adduct showed the strongest co
rrelation with 4-aminobiphenyl-hemoglobin adduct levels. These data su
ggest that noninvasive techniques can be applied to the study of carci
nogen-DNA adducts in the target organ of humans at risk for urinary bl
adder cancer.