RECOMBINANT A17 LYS HUMAN INSULIN - PURIFICATION AND CHARACTERIZATION

Recombinant A17 Lys human insulin precursor expressed in yeast cells w as isolated from the culture medium. After purification, the precursor was converted into A17 Lys human insulin. A17 Lys human insulin can b e crystallized but has much lower receptor-binding and biological acti vities than porcine or human insulin where A17 is Glu. It is proposed that the A17 Glu residue may be involved in the receptor-binding or th e active site of human insulin.