DNA ADDUCTS IN PERIPHERAL-BLOOD LYMPHOCYTES FROM ALUMINUM PRODUCTION PLANT WORKERS DETERMINED BY P-32 POSTLABELING AND ENZYME-LINKED-IMMUNOSORBENT-ASSAY

P-32-Postlabeling analysis and enzyme-linked immunosorbent assay (ELIS A) have been used to detect DNA adducts in peripheral blood lymphocyte s from primary aluminum production plant workers who were exposed occu pationally to a mixture of polycyclic aromatic hydrocarbons (PAHs). Pr eliminary results reported hem are from a comparative study being perf ormed in two aluminum plants. The levels of aromatic DNA adducts have been determined by the P-32-postlabeling assay in samples collected on two occasions, 1 year apart. PAH-DNA adduct levels have also been det ermined by competitive ELISA in the second set of DNA samples. The res ults show the necessity of follow-up biomonitoring studies to detect p ossible alterations in biological effect induced by changing exposures . The comparison of the results obtained by P-32-postlabeling and ELIS A may lead to a better understanding of the power and weaknesses of th e two methods applied in these studies.