Continuous surveillance of influenza was carried out in Pune between 1
978 and 1990. Most of the cases were identified during investigation o
f 16 outbreaks of influenza in Pune over this period. The majority of
cases were children. Ten of the outbreaks occurred during rainy season
s. A total of 290 isolates consisting of several antigenic variants of
influenza type A (H3N2), type A (H1N1), and type B viruses were isola
ted from throat/nasal swabs that were processed in chick embryos and M
DCK cell culture and identified using the haemagglutination inhibition
test. These variants circulated every year or in alternate years. Nea
rly two-thirds of the influenza virus isolates (181 out of 290) were f
rom children aged <10 years. Seasonal analysis indicated that the high
est number of isolates (174) were collected during the rainy months of
July, August and September, with the maximum number (93) in July.