D. Barber et al., SPECIFIC DEPLETION OF THE HOUSE-DUST MITE ALLERGEN DER-P-1 BY CEREAL FLOUR PROLAMINS, Journal of allergy and clinical immunology, 97(4), 1996, pp. 963-965
Background: Quantitation of Der 1 and Der 2 in dust samples by specifi
c monoclonal antibodies is a method used increasingly to evaluate mite
allergen exposure. The level of Der I has been proposed as a risk fac
tor for sensitization Aim: We report a drastic deer-ease in the Der 1/
Der 2 ratio when? dust samples are collected in bakeries. Methods: I W
heat pour and purified mites were extracted simultaneously: levels of
Dei p 1 and Der p 2 and cysteine protease activity were determined by
ELISA and inhibition experiments. Results: High titers of Der 2, but o
nly, trace amounts of Der p 1, were detected in dust samples collected
from bakeries. Both the level and proteolytic activity of Der p I app
eared greatly decreased when mites and wheat flour were coextracted. C
onclusions: Group 1 protein was found to be masked by flour components
, resulting in an underestimation of the mite content in baker dust. T
his problem, was not found for group 2 allergen.