SENSITIVITY TO TAXOID DERIVATIVES OF A NEWLY ESTABLISHED HUMAN ENDOMETRIOID OVARIAN ADENOCARCINOMA RADIORESISTANT CELL-LINE

An endometrioid ovarian adenocarcinoma cell line CAVEOC-2 was characte rized. Maintained in monolayered culture, CAVEOC-2 cells exhibited a 3 3-hr doubling time. When xenografted into nude mice, these cells produ ced fast growing tumors. Colony-forming efficiency in agar was 50%. DN A index was 1.5 and cytogenetic analysis showed a triploid karyotype. CAVEOC-2 cells did not express mdr-1 gene and were chemosensitive to d oxorubicin (IC50 = 1.82+/-0.76 mu mol/l), paclitaxel (IC50 = 3.33+/-0. 26 nmol/l) and docetaxel (IC50 = 0.68+/-0.28 nmol/l), while they showe d an intermediate sensitivity to cisplatin (IC50 = 9.40+/-1.02 mu mol/ l). CAVEOC-2 cells seemed highly radioresistant (SF2 = 0.81, alpha = 0 .02 Gy(-1), beta = 0.025 Gy(-2), and MID = 4.31 Gy). Activities of glu tathione S transferase and gamma-glytamyl transpeptidase were respecti vely 23.5- and 3.4- fold higher than those of sensitive A2780 cell lin e. These characteristics make the CAVEOC-2 cells a suitable model for the study of human endometrioid ovarian adenocarcinoma.