MICROWAVE-ENHANCED IMMUNOHISTOCHEMICAL STAINING OF A FORMALIN-SENSITIVE ANTIGEN - LAMININ RECEPTOR

Citation
Ec. Chew et al., MICROWAVE-ENHANCED IMMUNOHISTOCHEMICAL STAINING OF A FORMALIN-SENSITIVE ANTIGEN - LAMININ RECEPTOR, Anticancer research, 16(1), 1996, pp. 353-357
Citations number
17
Categorie Soggetti
Oncology
Journal title
ISSN journal
02507005
Volume
16
Issue
1
Year of publication
1996
Pages
353 - 357
Database
ISI
SICI code
0250-7005(1996)16:1<353:MISOAF>2.0.ZU;2-7
Abstract
Many potentially useful antigens have been difficult to detect in form alin-fixed, paraffin-embedded tissues. Recently a number of pathologic al and research laboratories have demonstrated that some antigens mask ed by formalin fixation could be restored to detectability by microwav e heating. Previously, we were unable to demonstrate laminin receptor in cells processed by the routine fixation, our results showed that mi crowave heating together with trypsin produced the best immunohistoche mical staining for this receptor. Nevertheless, no significance was fo und in the levels of 67 kD LR in high and low metastatic tumor cell li nes.