Thirty-one children, 2.6-12.2 years (mean = 7.9 +/- 5.0) were divided
into four clinical categories: normal controls (n = 7), recurrent tons
illitis (n = 8), idiopathic tonsillar hyperplasia (n = 9) and recurren
t tonsillitis with hyperplasia (n = 7). Immunohistopathologic studies
were performed to determine the presence and location of HLA-DR, S-100
positive antigen presenting dendritic cells (DCs) in six microanatomi
c compartments: surface and crypt epithelium and submucosae, extrafoll
icular areas and lymphoid follicles. The results were analyzed in the
context of the clinical history, bacteriology, and routine histopathol
ogy. The distribution of DCs was altered significantly with disease-fe
wer numbers of DCs were found in the surface epithelium and greater nu
mbers in the crypts and extrafollicular areas, than in normals, p < 0.
01. The surface:crypt ratio of DCs was 1 : 1 for normal tonsils, but d
ropped to 1 : 3 in disease. Chronic cryptitis was moe common in diseas
e and correlated strongly to the presence of beta-lactamase producing
microorganisms (R = 1.0). An increased total bacterial concentration w
as correlated with increased numbers of DCs in the surface epithelium
(R = 0.5, p < 0.009). In summary, the microanatomical distribution of
DCs within the tonsil is significantly altered in disease. These alter
ations appear to be influenced by potentially pathogenic bacteria more
often found in the crypts of abnormal tonsils.