RAPID-DETERMINATION OF AMPHOTERICIN-B IN SERUM AND URINE BY 3RD-ORDERDERIVATIVE SPECTROPHOTOMETRY

A derivative spectrophotometric method for rapid monitoring of amphote ricin B in serum and urine down to 30 ng/mL is described. Samples are treated with acetonitrile, and amphotericin B is directly quantified i n the crude extracts on the basis of the intensity of the peak that ap pears at 402 nm when the normal absorption spectrum is submitted to th ird-order derivative processing. Accuracy data suggested recoveries in the range of 84.3-94.9% for serum and 85.6-93.4% for urine. The preci sion of the method was better than 11.3% for serum and 9.2% for urine when samples contained as low as 29.6 ng/mL of amphotericin B. Ease of applicability, short analysis time, low cost, and reliability are the main advantages of the method.