MODELING OF SOLVENT EFFECTS IN THE ACTIVATION OF THE LIPASE FROM RHIZOMUCOR-MIEHEI

Authors
BENEDETTI F BERTI F LINDA P MIERTUS S SABOT A
Citation
F. Benedetti et al., MODELING OF SOLVENT EFFECTS IN THE ACTIVATION OF THE LIPASE FROM RHIZOMUCOR-MIEHEI, Bioorganic & medicinal chemistry letters, 6(7), 1996, pp. 839-844
Citations number
9
Categorie Soggetti
Chemistry Inorganic & Nuclear","Chemistry Medicinal
Journal title
Bioorganic & medicinal chemistry letters → ACNP
ISSN journal
0960894X
Volume
6
Issue
7
Year of publication
1996
Pages
839 - 844
Database
ISI
SICI code
0960-894X(1996)6:7<839:MOSEIT>2.0.ZU;2-U
Abstract
the effects of water and hydrophobic solvents on the stability of the open and closed forms of R. miehei lipase were evaluated with differen t solvent models. Desolvation of arginine 86 at the water-lipid interf ace plays a key role in the activation process, while the contribution from hydrophobic stabilization of the open form is less important. Co pyright (C) 1996 Elsevier Science Ltd