Mt. Zamarro et al., RECOVERY OF CELLULOLYTIC ENZYMES FROM WHEAT-STRAW HYDROLYSIS BROTH WITH DIOCTYL SULPHOSUCCINATE ISOOCTANE REVERSE MICELLES/, Biotechnology and applied biochemistry, 23, 1996, pp. 117-125
Hydrolysis of wheat straw was carried out with cellulases and hemicell
ulases from Trichoderma reesei QM 9414 cultures, The recovery of cellu
lolytic enzymes from hydrolysis broth was assayed through a forward tr
ansfer to dioctyl sulphosuccinate (AOT)/iso-octane reverse micelles an
d a backward transfer to a fresh aqueous medium, Neither reducing suga
rs nor DNA were recovered, but we detected beta-glucosidase, beta-xylo
sidase and xylanase activities in the final aqueous medium, We studied
the dependence of protein recovery on the pH of hydrolysis broth, the
AOT concentration, and the w(Oi) (molar ratio of water to AOT) of the
reverse micelles as well as the temperature of both transfers and the
ionic strength of the aqueous medium in the backward transfer, High t
emperature increased the recovery of enzymes, whereas the optimum pH v
aried for each enzyme. Moreover, the water content of the organic medi
um was titrated in each experiment, The hydrodynamic radius of the rev
erse micelles was higher when protein was solubilized. Under optimum t
ransfer conditions, 21.5% of beta-xylosidase and 7.5% of beta-glucosid
ase activity was recovered.