COMPARISON OF CONVENTIONAL AND CAPILLARY ELECTROSPRAY LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY AND LIQUID-CHROMATOGRAPHY TANDEM MASS-SPECTROMETRY FOR THE DETECTION OF 2'-DEOXYNUCLEOSIDE BISPHENOL-A DIGLYCIDYL ETHER ADDUCTS

Capillary liquid chromatography (LC) was coupled to electrospray mass spectrometry (MS) in order to improve the absolute detection limits re quired for the analysis of DNA adducts. The capillary LC/MS system was compared with conventional LC/MS system using 2'-deoxyadenosine (dAdo ). The absolute detection limit could be improved by a factor 177, lea ding to the detection of 600 fg of dAdo under single-ion monitoring (S IM) conditions. Using capillary LC/MS, 1 pg of a bisphenol A diglycidy l ether-dAdo addut could be detected under SIM conditions. Full-scan l ow-energy collision-activated decomposition (CAD) spectra of 550 pg ad duct are presented. Capillary LC/MS and capillary LC/MS/MS data are pr esented for in vitro reaction mixtures of 2'-deoxynucleosides and bisp henol A diglycidyl ether, a potent carcinogen.