Hn. Poinar et al., ULTRASTRUCTURE OF 30-40-MILLION-EAR-OLD LEAFLETS FROM DOMINICAN AMBER(HYMENAEA-PROTERA, FABACEAE, ANGIOSPERMAE), Experientia, 52(4), 1996, pp. 387-390
Hymenaea protera leaflet fossils entombed in amber, dated at 30 to 40
million years (mine strata and exomethylene dating) were observed by b
oth light and transmission electron microscopy. Ultrastructure preserv
ation in these leaflets shows the presence of chloroplasts with thylak
oid membranes, cell walls, mitochondria with associated endoplasmic re
ticulum, nuclei, and xylem tissue. Tissues show varying degrees of deg
radation; however, natural resin, which has perfused the cells, seems
to maintain the structural integrity of the membranes and walls. We co
nclude that preservation of amber entombed organisms results from dehy
dration and slow fixative properties leaving the ultrastructure in exc
ellent condition. These findings parallel reports on the exceptional p
reservation of amino acids and of DNA in amber-entombed organisms.