CLONING, SEQUENCING, AND EXPRESSION OF A GENE FROM CAMPYLOBACTER-JEJUNI ENCODING A PROTEIN (OMP18) WITH SIMILARITY TO PEPTIDOGLYCAN-ASSOCIATED LIPOPROTEINS

Authors
KONKEL ME MEAD DJ CIEPLAK W
Citation
Me. Konkel et al., CLONING, SEQUENCING, AND EXPRESSION OF A GENE FROM CAMPYLOBACTER-JEJUNI ENCODING A PROTEIN (OMP18) WITH SIMILARITY TO PEPTIDOGLYCAN-ASSOCIATED LIPOPROTEINS, Infection and immunity, 64(5), 1996, pp. 1850-1853
Citations number
27
Categorie Soggetti
Immunology,"Infectious Diseases
Journal title
Infection and immunity → ACNP
ISSN journal
00199567
Volume
64
Issue
5
Year of publication
1996
Pages
1850 - 1853
Database
ISI
SICI code
0019-9567(1996)64:5<1850:CSAEOA>2.0.ZU;2-R
Abstract
A Campylobacter jejuni genomic plasmid library was screened with antis erum generated against whole C. jejuni, revealing two immunoreactive c lones. Sequence analysis of the recombinant plasmids revealed a common open reading frame of 498 nucleotides encoding a protein of 165 amino acids with a calculated molecular mass of 18,018 Da. The recombinant product partitioned to the outer membrane fractions of Escherichia col i transformants and has been designated Omp18. The deduced amino acid sequence of the cloned C. jejuni gene exhibits considerable similarity to peptidoglycan-associated lipoproteins from other gram-negative bac teria.