EXPOSURE TO ENVIRONMENTAL TOBACCO-SMOKE RESULTS IN AN INCREASED PRODUCTION OF (-ANTI-BENZO[A]PYRENE-7,8-DIHYDRODIOL-9,10-EPOXIDE IN JUVENILE FERRET LUNG HOMOGENATES())
Rk. Sindhu et al., EXPOSURE TO ENVIRONMENTAL TOBACCO-SMOKE RESULTS IN AN INCREASED PRODUCTION OF (-ANTI-BENZO[A]PYRENE-7,8-DIHYDRODIOL-9,10-EPOXIDE IN JUVENILE FERRET LUNG HOMOGENATES()), Journal of toxicology and environmental health, 47(6), 1996, pp. 523-534
Six-week-old ferrets were exposed head-only to clean air or environmen
tal tobacco smoke (ETS) at an average particulate concentration of 38
+/- 13 mg/m(3) for 2 h/d, 5 d/wk for up to 15 wk. Twenty-four hours af
ter the last exposure, the ferrets were sacrificed and the metabolism
of benzo[a]pyrene and (-)-7R-trans-benzo[a]pyrene-7,8-dihydrodiol was
studied in lung homogenates. The results show that after ETS exposure
total metabolism of benzo[a]pyrene, measured by the accumulation of he
xane nonextractable radioactivity, was increased by 35% in the males a
nd 66% in the females (p <.05), respectively, of that observed with ai
r-exposed controls. With (-)-7R-trans-benzo[a]pyrene-7,8-dihydrodiol a
s substrate, the formation of both benzo[a]pyrene-r-7,t-8,9,c-10-tetra
hydrotetraol and enzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide-derived te
traols by lung homogenates of ETS-exposed male and female ferrets was
significantly increased compared to the air-exposed controls (p <.01).
DNA-bound radioactivity was significantly increased in both the males
(p <.01) and females (p <.05) compared to the air-exposed ferrets.